Genetic interaction between DNA repair factors PAXX , XLF, XRCC4 and DNA‐PKcs in human cells
نویسندگان
چکیده
منابع مشابه
A human XRCC4–XLF complex bridges DNA
DNA double-strand breaks pose a significant threat to cell survival and must be repaired. In higher eukaryotes, such damage is repaired efficiently by non-homologous end joining (NHEJ). Within this pathway, XRCC4 and XLF fulfill key roles required for end joining. Using DNA-binding and -bridging assays, combined with direct visualization, we present evidence for how XRCC4-XLF complexes robustly...
متن کاملSpecific Roles of XRCC4 Paralogs PAXX and XLF during V(D)J Recombination
Paralog of XRCC4 and XLF (PAXX) is a member of the XRCC4 superfamily and plays a role in nonhomologous end-joining (NHEJ), a DNA repair pathway critical for lymphocyte antigen receptor gene assembly. Here, we find that the functions of PAXX and XLF in V(D)J recombination are masked by redundant joining activities. Thus, combined PAXX and XLF deficiency leads to an inability to join RAG-cleaved ...
متن کاملInteractome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway
Non-homologous end joining (NHEJ) is a major pathway to repair DNA double-strand breaks (DSBs), which can display different types of broken ends. However, it is unclear how NHEJ factors organize to repair diverse types of DNA breaks. Here, through systematic analysis of the human NHEJ factor interactome, we identify PAXX as a direct interactor of Ku. The crystal structure of PAXX is similar to ...
متن کاملRobust DNA repair in PAXX‐deficient mammalian cells
To ensure genome stability, mammalian cells employ several DNA repair pathways. Nonhomologous DNA end joining (NHEJ) is the DNA repair process that fixes double-strand breaks throughout the cell cycle. NHEJ is involved in the development of B and T lymphocytes through its function in V(D)J recombination and class switch recombination (CSR). NHEJ consists of several core and accessory factors, i...
متن کاملPAXX promotes KU accumulation at DNA breaks and is essential for end-joining in XLF-deficient mice
Non-homologous end-joining (NHEJ) is the most prominent DNA double strand break (DSB) repair pathway in mammalian cells. PAXX is the newest NHEJ factor, which shares structural similarity with known NHEJ factors-XRCC4 and XLF. Here we report that PAXX is dispensable for physiological NHEJ in otherwise wild-type mice. Yet Paxx-/- mice require XLF and Xlf-/- mice require PAXX for end-ligation. As...
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ژورنال
عنوان ژورنال: FEBS Open Bio
سال: 2019
ISSN: 2211-5463,2211-5463
DOI: 10.1002/2211-5463.12681